Neuroprotective Effect of p38 MAPKs Inhibitor (SB239063) and Vitamin B12 against Lipopolysaccharide Induced Neuroinflammation

Abstract

Objectives: To evaluate the effect of MAP kinase inhibitor (SB239063) on neuroinflammation along with Vitamin B₁₂. Materials and Methods: Male wistar albino rats divided in to eight groups each group having six animals. Neuroinflammation was induced in animals by the lipopolysaccharides/ LPS (100μg/ml) fallowed by treatment with MAPK inhibitor (SB239063) alone (5mg/kg) and along with Vit B₁₂ (0.5mg/kg) for 30 days. Subsequently, animals were screened in the behavioral tests by using radial arm apparatus and actophotometer apparatus followed by biochemical and histopathological estimations. Results: Chronic LPS, 100 μg/kg i.p., caused physical and mental abnormalities in animals, including memory impairment, reduced locometer activity abnormal posture and cognitive deficit. Biochemical analysis of brain homogenate in LPS treated rats showed altered biochemical marker. Neurohistological alterations of various parts of brain of LPS treated rats exhibit severe neuronal space, irregular damaged cells. MAP Kinase inhibitor (SB239063) alone and along with Vit B₁₂ administered for 15 days significantly improved motor performance and cognitive behavior task and restored the histopathological changes. Conclusion: MAP Kinase inhibitor (SB239063) alone show significant effect at dose 5 mg/kg, I.V and whenever it was used in combination at reduced concentration with Vitamin B₁₂ provided slow neuroprotective effect.