Validated HPTLC Method to Estimate Silymarin in Marketed Herbal Formulation

Sharada L Deore; Hrutuja P Tidke; Somshekhar S Khadabadi; Bhushan A Baviskar; Anajli A Kide; Bhavna P Shende

doi:10.5530/ijpi.2021.1.10

Sharada L Deore Government College of Pharmacy, Amravati, Maharashtra, INDIA.
Hrutuja P Tidke Government College of Pharmacy, Amravati, Maharashtra, INDIA.
Somshekhar S Khadabadi Government College of Pharmacy, Amravati, Maharashtra, INDIA.
Bhushan A Baviskar Government College of Pharmacy, Amravati, Maharashtra, INDIA.
Anajli A Kide Government College of Pharmacy, Amravati, Maharashtra, INDIA.
Bhavna P Shende Government College of Pharmacy, Amravati, Maharashtra, INDIA.

DOI: https://doi.org/10.5530/ijpi.2021.1.10

Keywords: Silymarin, Hepatoprotective formulation, HPTLC, ICH, Precision

Abstract

Objectives: The objective of present research work is to develop simple, rapid and accurate high performance thin layer chromatographic method for quantitative estimation of silymarin in marketed hepatoprotective formulation. Methods: The separation was performed on silica gel 60 F₂₅₄ precoated TLC aluminum plates and toluene: ethyl acetate: formic acid: methanol as mobile phase. Determination and quantitation were performed by densitometric scanning at 288 nm. Validation of method is carried out as per International Conference of Harmonization (ICH) guidelines. Results: Accuracy, precision and recovery were all within the required limits. Conclusion: In conclusion, the developed HPTLC method is precise and accurate for implementation in routine quality control. The selected marketed hepatoprotective formulation contains the sufficient amount of the active ingredient which is required for its therapeutic efficiency.

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