Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone]

Abstract

Aim: A new simple, accurate, precise, Reverse Phase High-performance Liquid Chromatography method was developed and validated for the estimation of newly synthesized (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] derivative of pyrazolone. Materials and Methods: The chromatogram was run through Luna 5μ C₁₈(2). 250 × 4.80 mm. 272817-7 HPLC column; mobile phase consisting of acetonitrile and water in the ratio 90:10 was pumped through the column at a flow rate of 0.8mL/min. Results: The optimized wavelength was 237 nm. The retention time of (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] was found to be 7.3 min. The percentage relative standard deviation was obtained as 0.3%. Conclusion: This method was accurate, precise, and sensitive; therefore, could be used for the quality control and bioanalytical evaluation of (4Z) 3 methyl 1 (4 nitrobenzoyl) 1H pyrazole 4,5 dione 4[(4fluorophenyl)hydrazone] in drug testing laboratory.