Physicochemical, Phytochemical and HPTLC Analysis of a Novel Combined Herbal Formulation

  • Mohamed Shiffa Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, INDIA.
  • M Aslam Department of Ilmul Advia (Pharmacology), Jamia Hamdard, New Delhi, INDIA.
  • M H Kazmi Department of Ilmul Advia (Pharmacology), National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, Telangana, INDIA.
  • Syed Mansoor Ali Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, INDIA.
  • Nazeem Fahamiya Department of Study in Unani, Institute of Indigenous Medicine, University of Colombo, Rajagiriya, SRI LANKA.
  • Mohammad Mahfuzul Haque Department of Biotechnology, Jamia Millia Islamia, Jamia Nagar, New Delhi, INDIA.
Keywords: Anti-inflammatory, Unani medicine, Cinnamomum zeylanicum, Alpinia galanga, Withania somnifera

Abstract

Background: Cinnamomum zeylanicum (Darchini), Alpinia galanga (Khulanjan), and Withania somnifera (Asgand) have been used in joint diseases by Unani scholars since long. The combination of these ingredients may be more effective than they are given alone as these drugs are having potent anti-inflammatory, antioxidant, analgesic and rejuvenating properties. As per the WHO guideline, standardization should be done before undertaking any experiment which claims safety and efficacy. Therefore, this study is aimed to establish the physicochemical and phytochemical quality standards for this novel combination of herbal formulation for future references. Methods: In present study, extractive values such as methanol extract, aqueous extract, chloroform extract and aqueous ethanol extract was obtained. Further, loss on drying, pH, ash values, fluorescence analysis, qualitative phytochemical analysis and HPTLC fingerprint profile were done. Results: Mean values of methanolic, water, chloroform, and aqueous ethanolic extracts were 10.4±0.77%, 13.0±1.3%, 2.3±0.26% and 15.28±1.18% respectively. Further, the total ash, acid insoluble ash, and water-soluble ash were 3.3±0.32%, 1.13±0.09%, and 1.72±0.2% respectively. pH of 1% extract was 5.57±0.16 and 5.37±0.32 for 10% extract. It was observed that the percentage of moisture content was 4.67±0.42%. Qualitative phytochemical analysis revealed that the extract contains carbohydrates, glycosides, phenolic compounds, flavonoids, proteins and amino-acids, alkaloids, and saponins. Further, HPTLC fingerprint revealed the presence of many plant metabolites and active ingredients. Conclusion: The data generated in this study have been reported for the first time for this novel drug combination, which could be used as a source for future studies.

Downloads

Download data is not yet available.
HPTLC fingerprint of hydroethanolic extract of the combined herbal formulation (a) at UV 366nm. (b) after derivatization with anisaldehyde sulphuric acid at 580nm.
Published
2022-03-08
How to Cite
1.
Shiffa M, Aslam M, Kazmi MH, Ali S, Fahamiya N, Haque M. Physicochemical, Phytochemical and HPTLC Analysis of a Novel Combined Herbal Formulation. ijpi [Internet]. 8Mar.2022 [cited 29Sep.2022];12(1):62-5. Available from: https://www.jpionline.org/index.php/ijpi/article/view/1093