|ORIGINAL RESEARCH ARTICLE
|Year : 2018 | Volume
| Issue : 1 | Page : 7-13
Design and investigation of metformin hydrochloride–Indion-254 complex for dispersible tablets
Arlene Stuart Villarroel1, Madan Mohan Gupta2, Lexley M Pinto Pereira3, Andanappa K Gadad4
1 Department of Pharmacology; School of Pharmacy, Faculty of Medical Sciences, The University of the West Indies, Trinidad and Tobago, West Indies
2 School of Pharmacy, Faculty of Medical Sciences, The University of the West Indies; Laboratory of Pharmaceutical Formulation Design and Development, School of Pharmacy Faculty of Medical Sciences, St. Augustine, Trinidad and Tobago, West Indies
3 Department of Pharmacology, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago, West Indies
4 School of Pharmacy, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago, West Indies
|Date of Web Publication||11-Jul-2018|
Dr. Madan Mohan Gupta
School of Pharmacy, Faculty of Medical Sciences, Building 39, Eric Willaims Medical Sciences Complex, Mount Hope, The University of the West Indies, Trinidad and Tobago
Source of Support: None, Conflict of Interest: None
Objective: Metformin is a first-line agent for the treatment of Type 2 diabetes. The taste of metformin hydrochloride drug powder is bitter and its formulation are orally used. The objective of this study was to make a complex of metformin hydrochloride with Indion-254 resin for the purpose of preparation of dispersible tablet.
Materials and Methods: This study formulated metformin hydrochloride–Indion-254 complexes with different proportion of resin with fixed amount of drug as well as mixed at varying speed which improved the flowability.
Results: The resulted complex was investigated by the help of Fourier Transform- Infrared Spectroscopy, differential scanning calorimetry, and X-ray diffraction which were used to confirm complexation and to differentiate its features from the individual ingredients and the physical mixtures of ingredients.
Conclusion: The formulated metformin hydrochloride–Indion-254 complex showed better drug loading as well as good release in mixed phosphate buffer in 200 ml.
Keywords: Complex, drug dissolution, Indion-254, metformin hydrochloride, taste masking
|How to cite this article:|
Villarroel AS, Gupta MM, Pereira LM, Gadad AK. Design and investigation of metformin hydrochloride–Indion-254 complex for dispersible tablets. Int J Pharma Investig 2018;8:7-13
|How to cite this URL:|
Villarroel AS, Gupta MM, Pereira LM, Gadad AK. Design and investigation of metformin hydrochloride–Indion-254 complex for dispersible tablets. Int J Pharma Investig [serial online] 2018 [cited 2018 Nov 15];8:7-13. Available from: http://www.jpionline.org/text.asp?2018/8/1/7/236381
| Introduction|| |
In formulation development, resin polymers, which are anionic or cationic, are used independently for a particular function or combined with pharmaceutical ingredients such as dextromethorphan, streptomycin, and metformin for an intended purpose as taste masking.,,,,,,, Metformin has a bitter taste and is mainly used for Type 2 diabetes but is also indicated for polycystic ovarian syndrome.,, In this study, a brand of sodium polystyrene sulfonate (Indion-254), a strong acid cationic resin, was used with metformin hydrochloride to produce a taste-masked dispersible complex, which will ultimately be used to prepare a formulation suitable for patients who have difficulty in swallowing a solid dosage form.
| Materials and Methods|| |
Metformin hydrochloride received as gift sample from Intas Pharmaceuticals, India, and other chemicals and solvents used were of analytical grades purchased from different suppliers of India.
Preparation of metformin hydrochloride–Indion-254 complex
Specified amount of resin as per [Table 1] was weighed and added to the 60 ml distilled water in beaker. The beaker was placed on the water bath and it was stirred on magnetic stirrer for 1½ h at 50°C. After this period of stirring, weighed amount of metformin hydrochloride was dissolved in mixture and stirred on magnetic stirrer for 24 h. After 24 h, mixture was filtered under vacuum and rinsed with distilled water. The obtained residue was dried at 50°C and it was analyzed for percentage drug loading using equation below:
|Table 1: Composition of various metformin hydrochloride-Indion®-254 complexes|
Click here to view
Where Ci= Initial concentration of metformin hydrochloride in seventy milliliters of mixture and Cf= Final concentration of filtrate.
Characterization of resin complex
Drug–resin physical mixture and the drug–resin complex of metformin hydrochloride and sodium polystyrene sulfonate (Indion-254) were characterized using Fourier transform-infrared spectroscopy (PerkinElmer precisely spectrum 400 spectrometer and the universal attenuated total reflectance sampling accessory), differential scanning calorimetry (DSC) (Setsys Evolution TG-TMA calorimeter), and X-ray diffraction methods.
Drug–resin complex evaluation
The metformin hydrochloride–Indion-254 complex was analyzed for particle size (SEM and microscope methods), flow properties, and drug release in different medium and volumes in dissolution apparatus-2 at 75 RPM.
| Results|| |
Characterization of metformin hydrochloride–Indion-254 complex
In [Figure 1]a, wavenumbers were at 3135.48, 1547.75, 1045.35, and 634.37/cm for metformin hydrochloride; in [Figure 1]b, wavenumbers were at 3437.65, 1641.03, 1181.28, 1031.86, 830.27, and 674.42/cm for Indion ®-254; and in [Figure 1]c, metformin hydrochloride and Indion physical mixture looks similar to the Indion graph but with sharper peaks as seen in Metformin. Wavenumbers were at 1179.60, 3415.70, 1028.67, and 674.94/cm and compared to the physical mixture graph [Figure 1]d, metformin hydrochloride and Indion resin complex, the complex graph demonstrated some peaks around the 1500 and between 3400 and 3300 wavenumber/cm. The wavenumbers include 3346.47, 1555.07, 1172.72, 1012.43, and 674.09/cm.
|Figure 1: Fourier transform-infrared spectroscopy of metformin hydrochloride and its complex with resin. (a) Fourier transform-infrared spectroscopy of metformin hydrochloride, (b) Fourier transform-infrared spectroscopy of Indion-254 resin, (c) Fourier transform-infrared spectroscopy of physical mixture of metformin hydrochloride and Indion-254 resin, (d) Fourier transform-infrared spectroscopy of the metformin hydrochloride-Indion®-254 resin complex|
Click here to view
In [Figure 2]a, the two peaks were at 237.89°C and 338.33°C with an onset at 234.17°C and 299.10°C, respectively; in [Figure 2]b, one peak was seen at 103.42°C; in [Figure 2]c, two peaks observed at 235.15°C and 100°C; and in [Figure 2]d, one peak noticed at 95.78°C was observed. All the above differential scanning calorimetry graphs demonstrated endothermic heat flow.
|Figure 2: Differential scanning calorimetry of metformin hydrochloride and its complex with resin differential scanning calorimetry (a) metformin hydrochloride, (b) differential scanning calorimetry Indion-254 resin, (c) physical mixture of metformin hydrochloride and Indion-254 resin, (d) metformin hydrochloride–Indion-254 resin complex|
Click here to view
In [Figure 3]a, the graph for metformin hydrochloride demonstrated sharp peaks along the X-axis (abscissa) from 10 to 502. Peaks were observed at approximate values of 12.5°, 13°, 14.5°, 22°, 25°, 27°, 27.5°, 28°, 33°, 34.75°, 35.8°, 36.3°, 37.5°, and 39.9° with minor peaks thereafter until 50°. The major peaks were at 22.75°, 18°, 29.75°, 23.5°, 31.5°, and 28.5°, respectively, in order of intensity/counts. In [Figure 3]b, the graph showed no significant sharp peaks as was seen for metformin hydrochloride; in [Figure 3]c, graph for the drug–resin complex appears similar to that of Indion-254 with no significant sharp peaks; and in [Figure 3]d, graph is similar to Indion-254; however, there were a number of sharp peaks noticed at various points including those at approximately 12.5°, 18°, 22.75°, 23.5°, 27°, 28°, 29°, 30°, 33°, 35°, 36°, 36.25° and 45°.
|Figure 3: X-ray powder diffraction of metformin hydrochloride and its complex with resin. X-ray powder diffraction (a) metformin hydrochloride, (b) Indion-254, (c) metformin hydrochloride–Indion-254 resin complex, (d) physical mixture of metformin hydrochloride and Indion-254 resin|
Click here to view
Analysis of metformin hydrochloride
The melting point for metformin hydrochloride began at 234.4°C ± 4.34°C and ended at 253.8°C ± 4.71°C. The thermal values obtained using the capillary tube and the DSC methods exhibited similar melting onset readings of 234.4 ± 4.34 for the former and 234.17 for the latter.
Drug–resin complex evaluation
The flow property of metformin HCl and its complex with Indion resin in different proportion (1:4, 1:6) was determined by the fixed funnel method and angle of repose was calculated. The observation was recorded in [Table 2].
The metformin–Indion-254 complexes demonstrated better flow properties than metformin alone, and the complex 1:6 showed better flowability than metformin hydrochloride and the complex of ratio 1:4.
Drug release studies of in different medium and volumes
The drug release of metformin HCl was determined in distilled water and phosphate buffer pH 6.8, and data were recorded in [Table 3].
|Table 3: Drug release of metformin hydrochloride in different media and conditions|
Click here to view
[Figure 4]a shows % cumulative drug release in distilled water (4 ml) using magnetic stirrer, [Figure 4]b shows % cumulative drug release in phosphate buffer pH 6.8 (4 ml) using magnetic stirrer. [Figure 4]a and [Figure 4]b shows that the drug release is slightly greater for DRC 1:4 than DRC 1:6 and also observed was more drug release in phosphate buffer pH 6.8 than in distilled water.
|Figure 4: Percentage cumulative drug release in different media and condition. Drug release study (a) of metformin HCl–Indion®-254 complex in distilled water, (b) metformin HCl–Indion-254 complex in buffer pH 6.8, (c) metformin HCl–Indion-254 complex in phosphate buffer pH 6.8, (d) metformin HCl–Indion-254 complex in simulated gastric fluid|
Click here to view
[Figure 4]c represents % cumulative drug release in phosphate buffer pH 6.8 (200 ml) in dissolution apparatus and [Figure 4]d represents % cumulative drug release in simulated gastric fluid pH 1.2 (200 ml) in dissolution apparatus. In [Figure 4]c and [Figure 4]d, DRC 1:4 released more metformin in phosphate buffer pH 6.8 than in simulated gastric fluid pH 1.2.
It was observed that the metformin HCl–Indion 1:4 complex demonstrated higher dissolution efficiency in water and phosphate buffer pH 6.8 than the 1:6 ratio complex. In addition, higher results were seen for complex 1:4 in 200 ml of phosphate buffer pH 6.8 compared to comparable volume of simulated gastric fluid pH 1.2.
| Discussion|| |
Changes in external factors were found to influence the drug-loading percentage of ion exchange resins and hence can be used to optimize this process. In this study, an increase in the speed of the magnetic stirrer increased the loading percentage of the 1:5 and 1:6 ratios. Other factors that can affect drug-loading percentage include stirring time, temperature, particle size, pH of the media, and type of method used. The unbounded drug in the filtrate is measured using ultraviolet-infrared spectroscopy or high-pressure liquid chromatography, and then, this value is subtracted from the initial concentration to determine the amount of drug that was bound to the resin.,,
When the complex is formed, it is physically and chemically different to a physical mixture of the individual components. These differences were observed in the graphs demonstrated for infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry. In the X-ray diffractogram, the physical mixture maintained some peaks that were seen also in the metformin hydrochloride graph; this indicated their crystalline structure. However, this was not observed for the resin or drug–resin complex graphs, confirming the presence of complexation through suitable bonding. In the complexed form, the bitter group, such as the amine group in metformin, binds to the resin and hence masks the bitter taste of the drug. Therefore, the physical mixture will not be as effective as the complex in taste masking because the physical mixture behaves as two independent substances as were seen in the calorimetry results where two distinct melting points were observed for the physical mixture as compared to one for the complex. For this study, taste-masking properties were tested by the dissolution/drug release method, which determines the concentration of the drug released in various media compared to the pure drug. According to the guidelines of the Federation International Pharmaceutique and American Association of Pharmaceutical Scientists, <10% of a drug released/dissolved in 5 min constitutes effective taste masking; nevertheless, this also depends on the bitterness extent/index/threshold of the respective drug. Therefore, if the bitterness threshold for metformin hydrochloride can be acquired, in terms of concentration, then this will verify whether the approximate 37% release of metformin hydrochloride observed for 4 ml of phosphate buffer pH 6.8 would provide any taste-masking effect.,,, Other probable methods consist of the electronic tongue or alternative automotive device that measures the bitter level of a drug in comparison to standard, in vivo, and ex vivo procedures. The taste-masking results for this study showed the complex to be more stable in water, a neutral medium, than in phosphate buffer pH 6.8, as less of the drug was released/dissociated from the resinate/complex opposed to pure drug in similar medium and volume, hence minimizing the bitter taste of metformin hydrochloride. Consequently, metformin hydrochloride is cationic and binds to the anionic resin to form the metformin HCl-Indion-254 complex; hence, the ions present in the media according to pH dissociate the drug from the complex.
More resin added increases the amount of binding sites available for the drug to bond, therefore higher the drug loading percentage. However, higher resin ratios are not suitable to formulate into a tablet as the resulting product will require to be too large in size. In our study, DRC 1:4 released more metformin in 4 ml of water and phosphate buffer pH 6.8 than DRC 1:6 as a result of the bonding extent at a higher loading percentage. In similar respect to its bonding, metformin hydrochloride was released more in phosphate buffer pH 6.8 than in simulated gastric fluid pH 1.2, which may explain why metformin is not released or is less absorbed in the stomach as compared to the small intestines. Furthermore, the results of the study demonstrated that with a larger volume of medium, the more metformin is released out of the complex as was observed with phosphate buffer pH 6.8 in 4 ml compared to 200 ml. It, therefore, is imperative to analyze the complex based on the formulation to be developed as an oral disintegrating product will require to be tested in a volume of usually 10 ml or less as oppose to a solid dosage form which will require a volume of 200 ml or more.
Particle size of the drug or resin was stated earlier to affect the drug-loading percentage of the complex; however, particle size can also influence the taste properties such as mouthfeel as well as the flow and manufacturing process. Finer particles have a greater surface area hence a faster dissolution but decreased flowability.,,, Direct compression is the pharmaceutical process favored by most industries compared to other methods such as wet granulation and requires good flow properties of the formulation blend which includes excipients. Hence, the excipients selected are to enhance this and other attributes of the formulation.
| Conclusion|| |
The formulated metformin hydrochloride-Indion-254 complex showed capabilities to provide taste-masking and disintegrating properties and hence can be developed into an oral- or water-disintegrating tablet.
Financial support and sponsorship
This study was financially supported by the University of the West Indies, St. Augustine, Trinidad and Tobago, WI.
Conflicts of interest
There are no conflicts of interest.
| References|| |
Elder DP. Pharmaceutical applications of ion-exchange resins. J Chem Educ 2005;82:575-87.
Jeong SH, Park K. Drug loading and release properties of ion-exchange resin complexes as a drug delivery matrix. Int J Pharm 2008;361:26-32.
Kim JI, Cho SM, Cui JH, Cao QR, Oh E, Lee BJ, et al. In vitro
and in vivo
correlation of disintegration and bitter taste masking using orally disintegrating tablet containing ion exchange resin-drug complex. Int J Pharm 2013;455:31-9.
Walsh J, Cram A, Woertz K, Breitkreutz J, Winzenburg G, Turner R, et al.
Playing hide and seek with poorly tasting paediatric medicines: Do not forget the excipients. Adv Drug Deliv Rev 2014;73:14-33.
Yewale CP, Rathi MN, Kore GG, Jadhav GV, Wagh MP. Formulation and development of taste masked fast-disintegrating tablets of chlorpheniramine maleate using ion-exchange resins. Pharm Dev Tech 2013;18:367-76.
Bhoyar P, Amgaonkar Y. Taste masking and molecular properties of metformin hydrochloride-indion 234 complexes. J Young Pharm 2011;3:112-8.
Marmwar PA, Singh MC. Modified release of metformin hydrochloride using ion exchange resin complex in floating mucoadhesive tablets. Asian J Pharm 2016;10:7-15.
Rashid NS, Muhammad A, Abdul M, Muhammad N, Hassali KA, Azmi M, et al
. Formulation development of metformin tablet and its comparative in vitro
study with different brands in Pakistan. Int J Pharm Sci Rev Res 2013;19:12-7.
Sahu R, Pany D, Pati D, Dash A. Design and evaluation of fast disintegrating tablets of metformin by effervescence method. Int J Pharm Biol Sci Arch 2011;2:1253-7.
Dasankoppa FS, Komal S, Sholapur HN, Nanjundaswamy NG, Sajjanar VM. Design, optimization and evaluation of chewable tablets of clarithromycin using ion exchange resins. Indian J Pharm Sci 2016;78:818-26.
Fu Y, Yang S, Jeong SH, Kimura S, Park K. Orally fast disintegrating tablets: Developments, technologies, taste-masking and clinical studies. Crit Rev Ther Drug Carrier Syst 2004;21:433-76.
Gupta MM, Patel V. Formulation and evaluation of oral dispersible tablet of cinnarizine. J Drug Deliv Ther 2013;3:12-7.
Gupta MM, Gupta N, Chauhan BS, Pandey S. Fast disintegrating combination tablet of taste masked levocentrizine dihydrochloride and monetilukast sodium: Formulation design development and evaluation. J Pharmaceutics 2014; ID 568320.
Garg A, Gupta MM. Taste masking and formulation development & evaluation of mouth dissolving tablets of levocetrizine hydrochloride. J Drug Deliv Ther 2013;3:123-30.
Goel H, Vora N, Rana V. A novel approach to optimize and formulate fast disintegrating tablets for nausea and vomiting. AAPS PharmSciTech 2008;9:774-81.
Bhise K, Shaikh S, Bora D. Taste mask, design and evaluation of an oral formulation using ion exchange resin as drug carrier. AAPS PharmSciTech 2008;9:557-62.
Roy GM. The applications and future implications of bitterness reduction and inhibition in food products. Crit Rev Food Sci Nutr 1990;29:59-71.
Gupta MM, Saini TR. Preformulation parameters characterization to design, development and formulation of vancomycin hydrochloride tablets for psudomembranous colitis. Int J Pharm Res Dev 2009;1:1-7.
Kaning K, Kanada K. Application of gel formation for taste masking. Chem Pharm Bull 1997;45:1063-8.
Devireddy SR, Gonugunta CS, Veerareddy PR. Formulation and evaluation of taste-masked levocetirizine dihydrochloride orally disintegrating tablets. PDA J Pharm Sci Technol 2009;63:521-6.
Sohi H, Sultana Y, Khar RK. Taste masking technologies in oral pharmaceuticals: Recent developments and approaches. Drug Dev Ind Pharm 2004;30:429-48.
Tanigake A, Miyanaga Y, Nakamura T, Tsuji E, Matsuyama K, Kunitomo M, et al.
The bitterness intensity of clarithromycin evaluated by a taste sensor. Chem Pharm Bull (Tokyo) 2003;51:1241-5.
Sharma V, Chopra H. Formulation and evaluation of taste masked mouth dissolving tablets of levocetirizine hydrochloride. Iran J Pharm Res 2012;11:457-63.
Puttewar TY, Kshirsagar MD, Chandewar AV, Chikhale RV. Formulation and evaluation of orodispersible tablet of taste masked doxylamine succinate using ion exchange resin. J King Saud Univ Sci 2010;22:229-40.
Lalji V, Gupta MM. Oral disintegrating tablet of antihypertensive drug. J Drug Deliv Ther 2013;3:85-92.
Xu J, Bovet LL, Zhao K. Taste masking microspheres for orally disintegrating tablets. Int J Pharm 2008;359:63-9.
Shukla D, Chakraborty S, Singh S, Mishra B. Mouth dissolving tablets: An overview of evaluation techniques. Sci Pharm 2009;77:327-41.
[Figure 1], [Figure 2], [Figure 3], [Figure 4]
[Table 1], [Table 2], [Table 3]