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 Table of Contents  
Year : 2011  |  Volume : 1  |  Issue : 3  |  Page : 148-156

Formulation and evaluation of sustained release bioadhesive tablets of ofloxacin using 3 2 factorial design

1 Department of Pharmaceutics, NANDHA College of Pharmacy, Erode, India
2 Department of Pharmaceutics, C. L. Baid Metha College of Pharmacy, Thorapakkam, Chennai, India
3 Department of Pharmaceutics, KK College of Pharmacy, Mangadu, Chennai, Tamil Nadu, India

Date of Web Publication12-Oct-2011

Correspondence Address:
Hemant H Gangurde
Department of Pharmaceutics, NANDHA College of Pharmacy, Erode, Tamilnadu
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2230-973X.85964

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Background: Oral sustained release gastroretentive dosage forms offer many advantages for drugs having absorption from upper gastrointestinal tract and improve the bioavailability of medications that are characterized by narrow absorption window. The aim of current study was to design sustained release bioadhesive gastroretentive dosage form of ofloxacin. Materials and Methods: A 3 2 full factorial design was employed to systematically study the drug release profile and bioadhesive strength. Carbopol 934P and HPMC K100M were selected as the independent variables. Compatibility between drug and polymer was tested by fourier transform infrared (FTIR) and X-ray diffraction (XRD) techniques. Tablets were prepared by direct compression and were evaluated for tablet characteristics, swelling study, adhesion strength, percent drug released, radiographic imaging study and stability study. The optimized formulation was then compared with marketed formulation (Oflin OD®). Results: Tablets prepared showed good tablet characteristics, optimum swelling property, and good adhesion strength with high detachment force. Most of the formulations including the optimized formulation followed Higuchi kinetics and the drug release mechanism was found to be anomalous. Radiographic image proved that tablet remains intact in its structural integrity and shape in stomach up to 24 h. The short-term accelerated stability testing was carried out for the optimized formulation, and results revealed that drug content, in-vitro dissolution and all other parameters were within acceptable limits. Conclusion: Thus, the prepared bioadhesive gastroretentive ofloxacin tablet may prove to be a potential candidate which increases the bioavailability of ofloxacin for any intragastric condition.

Keywords: Bioadhesion, gastroretentive dosage form, ofloxacin, radiographic imaging study

How to cite this article:
Gangurde HH, Chordiya MA, Tamizharasi S, Senthilkumaran K, Sivakumar T. Formulation and evaluation of sustained release bioadhesive tablets of ofloxacin using 3 2 factorial design. Int J Pharma Investig 2011;1:148-56

How to cite this URL:
Gangurde HH, Chordiya MA, Tamizharasi S, Senthilkumaran K, Sivakumar T. Formulation and evaluation of sustained release bioadhesive tablets of ofloxacin using 3 2 factorial design. Int J Pharma Investig [serial online] 2011 [cited 2019 Jun 16];1:148-56. Available from:

  Introduction Top

Oral sustained (SR) systems continue to be the most popular ones among all the drug delivery systems. [1] Bioadhesive delivery systems offer several advantages over other oral SR systems by virtue of prolongation of residence time of drug in gastrointestinal (GI) tract, and targeting and localization of the dosage form at a specific site. Also, these bioadhesive systems are known to provide intimate contact between dosage form and the absorptive mucosa, thereby resulting in high drug flux through the absorbing tissue. [2],[3],[4],[5],[6] Prolonging the gastric retention of a delivery system is sometimes desirable for achieving therapeutic benefit of drugs that are absorbed from the proximal part of the gastrointestinal tract (GIT) or that are less soluble in or are degraded by the alkaline pH they encounter at the lower part of GIT. Gastroretentive drug delivery system (GRDDS) is thus beneficial for such drugs by improving their bioavailability, therapeutic efficacy and by possible reduction of dose. Apart from these advantages, these systems offer various pharmacokinetic advantages like maintenance of constant therapeutic levels over a prolonged period and thus reduction in fluctuation in therapeutic levels, minimizing the risk of resistance, especially in case of antibiotics. [7]

Ofloxacin is an antibacterial antibiotic. The mechanism by which it exerts this effect is by binding to and inhibiting topoisomerase II (DNA gyrase) and topoisomerase IV. These bacterial enzymes are responsible for the coiling and uncoiling of DNA, which is needed for bacterial cell repair and replication. Ofloxacin exhibits pH-dependant solubility. It is more soluble in acidic pH and slightly soluble at neutral or alkaline pH conditions (intestinal environment). The molecule exists as a zwitterions at the pH conditions in the small intestine and is easily absorbed there. [8],[9]

The objective of the present work was to develop gastroretentive formulation, which releases drug in the stomach and upper GIT, and form an enhanced opportunity of absorption in the stomach and upper GIT rather than the lower portion of the GIT. Our work includes development of bioadhesive gastroretentive formulation of ofloxacin, and evaluation of tablet characteristics, swelling study, adhesion strength, percent drug release, radiographic imaging study and stability study. Hence, an attempt was made to develop GRDDS of ofloxacin which would increase the bioavailability of ofloxacin.

  Materials and Methods Top


Ofloxacin and Carbopol 934P were kindly gifted by Madras Pharmaceutical Ltd., Chennai. Hydroxypropylmethyl cellulose (HPMC K100M), potassium dihydrogen phosphate, and lactose were procured from Loba Chemical, Mumbai. All the other chemicals used were of analytical grade.


Identification of drug

Ultraviolet spectroscopy

25 μg/ml stock solutions of ofloxacin were prepared by weighing 25 mg of ofloxacin and dissolving in 1 l of different solvents (i.e. 0.1 N HCl, water and phosphate buffer, pH 6.8). Then, ultraviolet (UV) spectrum of 25 μg/ml solution of the ofloxacin was recorded in the range of wavelengths from 200 to 400 nm using UV-visible double beam spectrophotometer (V630, Jasco) [Table 1] and [Figure 1]. Fourier transform infrared analysis. [10],[11]
Table 1: λmax of ofloxacin in different solvents

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Figure 1: UV spectra of ofloxacin in 0.1 N aqueous HCl solution (---), in water (---) and in phosphate buffer solution, pH 6.8(---)

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Infrared spectroscopic analysis of ofloxacin [Figure 2] and [Table 2] was performed using Fourier transform infrared (FTIR) spectrophotometer 4100 (Jasco), with a resolution of 8 cm−1 , in the range of 4000-400 cm−1 , on a zinc selenide (ZnSe) crystal.
Table 2: FTIR analysis of the drug

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Figure 2: FTIR spectra of ofloxacin

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Compatibility study of drug with polymers

FTIR analysis

Each polymer used in the formulation was mixed with drug at levels that are realistic with respect to the final dosage form. Drug-polymer mixtures were stored at 40°C and 75% relative humidity (RH) for a 3-month period. After 3 months, each mixture was tested for its stability by FTIR spectroscopy [Figure 3], [Figure 4] and [Figure 5]. [10],[11]
Figure 3: FTIR spectra of Carbopol 934P

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Figure 4: FTIR spectra of HPMC K100M

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Figure 5: Overlay FTIR spectra of ofloxacin and polymer mixture

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Drug, polymers and their mixture (tablet triturated after compression) were analyzed by X-ray diffraction (XRD) in order to verify the effect of compression on crystallinity of components as well as to test any interaction between the excipients. Powder XRD patterns were obtained with an X-ray diffractometer (Model-MenifleX) at the following test conditions: time per step, 0.400 sec; step size, 2 = 0.020° (is incident angle); current, 30 mA at 40 kV; CuK rays (wavelength = 1.542 Å) [Figure 6], [Figure 7], [Figure 8] and [Figure 9].
Figure 6: XRD spectra of ofloxacin

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Figure 7: XRD spectra of HPMC K100M

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Figure 8: XRD spectra of Carbopol 934P

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Figure 9: XRD spectra of the ofloxacin and polymer mixture

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Factorial design

A 3 2 randomized full factorial design was applied in the present study. In this design, 2 factors were evaluated, each at 3 levels, and experimental trials were performed at all the 9 possible combinations. The amount of bioadhesive polymer, Carbopol 934P (X1), and the amount of release modifying polymer, HPMC K100M (X2), were selected as independent variables. The swelling index, bioadhesion strength, time required for 100% drug release and cumulative % drug released in 12 h, and mean dissolution time (MDT) were selected as dependent variables. The formulations using 3 2 randomized full factorial designs are as shown in [Table 3] and [Table 4]. [13],[14],[15]
Table 3: Formulations using 32 randomized full factorial design

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Table 4: Coded formulation

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Preparation of tablets

Drug ofloxacin and other excipients were passed through sieve #60 mesh separately and then all ingredients mixed uniformly. Tablets were prepared by direct compression method using rotary press (Rimek, Mumbai India). Compression force for all the tablets was adjusted to get tablets of hardness 6-7 kg/cm 2 . Hardness was measured by Monsanto hardness tester.

Characterization of powder blends of drug and excipients

The powder blend of formulations shown in [Table 5] were evaluated for flow properties as follows and observations are reported in [Table 6]. [16]
Table 5: Formulation as per 32 factorial design

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Table 6: Evaluation parameters of formulation F1-F9 powder blends

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Evaluation of tablets

Tablet characteristics

Formulated matrix tablets were evaluated for thickness by using Digital Vernier caliper (Mitutoyo), weight variation, hardness (Monsanto hardness tester), friability (Roche friabilator) and drug content. [17]

Drug content: Three tablets were weighed and finely powdered. The quantity equivalent to 400 mg of ofloxacin was weighed accurately and taken in a 100-ml volumetric flask. Fifty milliliters of 0.1 N HCl was added, sonicated for 30 min, made up to 100 ml with 0.1 N HCl and filtered. Two milliliters of the above solution was diluted to 100 ml in a volumetric flask and the drug concentration was determined at 293.8 nm by using UV spectrophotometer.

Swelling measurements

Swelling was measured by placing pre-weighed specimen tablets (W1) into the Type-I dissolution apparatus containing 900 ml of 0.1 N HCl at 37 ± 0.5°C. The medium was stirred continuously at 100 rpm. At different time intervals, the specimens were removed, wiped gently with a tissue paper to remove surface water and weighed (W2). Swelling index was determined using the equation:

Swelling index = (W2 − W1) × 100/W1. [18]

Ex-vivo mucoadhesion measurement

Mecmesin Ultra tester (detachment force): Ex-vivo mucoadhesion studies were carried out on goat gastric mucosa. In evaluation of adhesion, it is important to use uniform surfaces that allow the formation of reproducible adhesive bonds. In the present study, goat gastric mucosa was used as model mucosal surface for bioadhesion testing. The goat stomach was cut into pieces and was mounted with mucus surface facing upward on wooden block specially prepared for holding mucosal tissues for bioadhesion testing. The wooden block was attached with double-sided adhesive tape to support the tissue on it. The thread was tied to firmly place the tissue on wooden block. The tablet was attached to the upper support using cynoacrylate glue [Figure 10]. The entire set was mounted on platform of test stand of Mecmesin Ultra Tester. Before the measurement, the mucus membrane was moistened with water. The upper support was lowered at a speed of 0.5 mm/sec until contact was made with the tissue at the predetermined force of 50 mN for a contact time of 5 min. Contact time is important because this period corresponds to a pre-swelling necessary for bioadhesive polymer chain disentanglement and establishment of intimate contact between polymer and mucin chains. At the end of contact time, the upper support was withdrawn at a speed of 0.5 mm/sec to detach the tablet from the membrane. The detachment force, i.e. the force required to separate tablet from tissue surface was reported as bioadhesive strength. The bioadhesive strength was reported in terms of miliNewton (mN). [18]
Figure 10: Mecmesin Ultra tester (detachment force)

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Dissolution study

The release of ofloxacin from tablet was studied using USP Type I Dissolution apparatus; the dissolution medium was 0.1 N HCl, pH 1.2, the volume being 900 ml, the rotation speed was 100 rpm, and the temperature was maintained at 37°C. [19]

Dissolution studies were carried out for 24 h. Samples of 5 ml were taken at an interval of every 2 h up to 24 h. After collecting the sample, the dissolution medium was replenished with the same sample volume of fresh medium. Sample was analyzed using UV spectrophotometer at 293.8 nm.

Data treatment [20],[21],[22] : The dissolution data were subjected to different model-dependent and -independent treatments.

Model-dependent methods

Zero-order kinetics: Drug dissolution from pharmaceutical dosage forms that do not disaggregate and release the drug slowly can be represented by the following equation:

Wo − Wt = Kt,

where Wo is the initial amount of drug in the pharmaceutical dosage form, Wt is the amount of drug in the pharmaceutical dosage form at time t and K is a proportionality constant. Dividing this equation by Wo and simplifying we get:

ft = K0t,

where ft = 1 - (Wt/Wo) and ft represents the fraction of drug dissolved in time t and K0 the apparent dissolution rate constant or zero-order release constant.

First-order kinetics: lnQt = lnQ0 - K1t,

where Q represents the fraction of drug released in time t and K1 is the first-order release constant.

Higuchi model

Qt = QHt 1/2 ,

where Qt represents the fraction of drug released in time t and QH is the Higuchi dissolution constant.

Korsmeyer-Peppas model

Q = Kpt n ,

where Kp is a constant incorporating the structural and geometric characteristics of the drug dosage form, n is the release exponent, indicative of the drug dissolved fraction at time t. This equation was further simplified and proposed by Ritger and Peppas as

Mt/M = Kt n ,

where Mt is the drug released at time t, M is the amount of drug released at infinite time, K is a kinetic constant, and n is the diffusional exponent.

The value of n indicates the drug release mechanism. For a slab, the value n = 0.5 indicates Fickian diffusion and values of n between 0.5 and 1.0 or n = 1.0 indicate non-Fickian mechanism. In case of a cylinder, the values are n = 0.45 instead of 0.5, and 0.89 instead of 1.0 [Table 7].
Table 7: Interpretation of diffusional release mechanism from polymeric films[20]

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Determination of bioadhesive property by radiographic imaging technique

The in vivo behavior of the gastroretentive bioadhesive tablet of optimized formulation F9 in the rabbit stomach was observed in real time using a radiographic imaging technique. A healthy male albino rabbit weighing 2 kg was used throughout the study. Also, to assess the formulation in vivo, a specialized formulation with barium sulfate was prepared, i.e. optimized formulation F9 using barium sulfate.

The prepared F9 formulation using barium sulfate was administered to the rabbit by intubation method. After that, 10-12 ml water was given immediately. During the experiment, the rabbit was not allowed to eat any food, but water was available ad libitum. Then, the radiographic images were taken at various time intervals. [23]

Stability study

The optimized formulated tablets were wrapped in aluminum foils and then were subjected to temperature of 40°C and 75% RH for a period of 1 month. The tablets were then analyzed for organoleptic characteristics, hardness, drug content and drug release. [24]

  Results and Discussion Top

Compatibility study of drug with polymers

FTIR analysis

The spectrum remained unchanged at the end of 3 months. The overlay spectra of the drug and the mixture are shown in [Figure 2], [Figure 3], [Figure 4] and [Figure 5]. Therefore, the active molecule is not altered by the addition of polymer substances. This study helps in assuming the stability of drug and in further development of dosage form.

XRD study

Ofloxacin was found to be crystalline in nature as it showed sharp peaks in X-ray diffraction spectrum, as shown in [Figure 6], [Figure 7], [Figure 8] and [Figure 9]. No sharp peaks were observed in the XRD spectra of Carbopol 934P and HPMC K100M; this indicates that both the polymers are amorphous in nature and this finding is in accordance with the literature reports. Peak intensities of Carbopol and HPMC K100M were less as compared to ofloxacin. The peak intensities of Carbopol and HPMC K100M were weak in the spectrum of the compressed mixture. This may be due to dilution of the polymers by drug since the drug composed 55% by weight of the whole mixture while the polymers were 40% by weight of the whole mixture.

Characterization of powder blends of drug and excipients

Particle size of drug and polymer affects flow characteristics, compression as well as release from delivery system. Individually drug powder, with a crystalline nature, has good flow property, but Carbopol and HPMC show poor to passable flow properties. But when mixed together with glidant, it shows passable to good flow property. The difference in compressibility of the mixture may be due to the difference in particle nature of ingredients [Table 6].

Tablet characteristics

Hardness of tablets was in the range of 6-7 kg/cm 2 . Percent weight loss in the friability test was found to be less than 0.4% in all the formulations. Content uniformity was found within 100 ± 2% of 400 mg of ofloxacin.

Determination of swelling index

The swelling index of all formulations was in the range of 111.44-241.20%. The study of swelling behavior of all formulations showed that swelling is increased up to 24 hours; but initially swelling is slow up to 6 hours and at the end of 24 hours swelling increases as the time increases. From the results, it is also seen that as the concentration of polymers increases, % swelling index also increases [Table 8] and [Figure 11].
Table 8: Swelling study of blended formulation

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Figure 11: Swelling index of formulations

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Ex-vivo mucoadhesion measurement

Mecmesin Ultra tester (detachment force)

The results of the detachment force of ofloxacin bioadhesive tablets are given in [Table 9]. In all the formulations, as the polymer concentration increases, the detachment force increases. Increasing the concentration of polymer may provide more adhesive sites and polymer chains for interpenetration with mucin, resulting consequently in the augmentation of bioadhesive strength. The polymer in the tablet first gets activated by moistening and gets adhered to mucus membrane and after that formation of intimate contact between mucus and polymer chains takes place leading to formation of bonds and strengthening the adhesive joints. Very strong bioadhesion could damage the epithelial lining of the mucosa, so the concentration of polymer should be optimum because high concentration may cause inflexible confirmation of polymer coils that cannot participate actively in adhesion with mucus macromolecule [Figure 12] and [Figure 13].
Table 9: Ex-vivo mucoadhesion measurement of blended formulation

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Figure 12: Force of detachment of formulations

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Figure 13: Adhesion time of formulations

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Dissolution study

Drug release profile

Carbopol and HPMC are hydrophilic polymers. When tablets containing these polymers come in contact with water, it allows gradual hydration of the tablet matrix, leading to swelling of the tablets. Water decreases the glass transition temperature of the polymers to the experimental temperature. At this temperature, glassy polymer is transformed into a rubbery state. Mobility of polymeric chains is enhanced in this state. This favors the transport of water into tablet and consequently transport of the dissolved drug from tablet core to the dissolution medium. Drug release from matrix tablet is determined by drug characteristics, delivery system and destination (site of drug release). Drug content of each tablet was 400 mg and 900 ml of dissolution medium was used for dissolution studies. Ofloxacin has a solubility of 76 mg/ml in 0.1 N HCl at 25°C. [1],[26] Maintaining sink condition is important during the dissolution experiment for consistent and accurate measurement of the dissolution rate. According to the United States Pharmacopeia (USP, 2005), sink condition is established when the saturation solubility is at least three times more than the drug concentration in the dissolution medium. Thus, sink conditions could be maintained throughout the dissolution study and drug solubility could not be a factor responsible for retardation of drug release from the formulations studied. Hence, retardation of drug release from the formulations could be attributed to the properties of polymers used in the formulations as shown in [Table 10] and [Figure 14]. [25],[26]
Table 10: Drug release profile of blended formulations

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Figure 14: Comparison of drug release of formulations F1-F9

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Data treatment

The correlation coefficient (R) value was used as criteria to choose the best model to describe drug release from the mucoadhesive sustained release tablets. The R-value in various models is given in [Table 11]. The R-values (R>0.9925) obtained for fitting the drug release data to the Higuchi equation, indicated that the drug release mechanism from these tablets was diffusion controlled.
Table 11: Correlation coefficient (R) values in various kinetic models tested

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Comparison: Optimized vs. Marketed

Dissolution profiles comparison of marketed product (Oflin OD) and optimized formulation is given in [Table 12] and [Figure 15]. Also Correlation Coefficient (R) Values in Various Kinetic Models Tested for marketed and optimized formulation shown in [Table 13].
Table 12: Comparison between optimized versus marketed formulation

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Table 13: Correlation coefficient (R) values in various kinetic models

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Figure 15: Comparison between optimized versus marketed formulation

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From the radiographic images in [Figure 16], it is proved that tablet remains intact in its structural integrity and shape in stomach. The changes in position of tablet in [Figure 16]b and c at 30 min and 1 h, respectively, provide the evidence of bioadhesive nature of tablets in rabbit's stomach. At 30 min and 1 h, the swelling of tablet is observed very clearly with centre core and a small swelling layer around it. So, it provides the swelling property of this formulation. Then, the unchanged position of tablet starts from 12 th h and is maintained throughout up to 24 th h, indicating the bioadhesive property of the developed formulation (i.e. it remained in the stomach for 24 h which may be due to bioadhesiveness). As the swelling continues, the core and outer swelling get diminished and size reduction along with slight modulation in structure at 24 th h indicates structural integrity retaining capacity of developed formulation in stomach for 24 h.
Figure 16: Photograph shows: (a) X-ray without tablet, (b) X-ray after 30 min administration of tablet, (c) X-ray after 1 h, (d) X-ray after 6 h, (e) X-ray after 12 h, (f) X-ray after 24 h

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Stability study of optimized formulation

The short-term-stability testing was carried out for the optimized formulation. The results for the dissolution profile are as depicted in the graph. Short-term accelerated stability data obtained for optimized formulation revealed that drug content, in-vitro dissolution and all other parameters were within acceptable limits [Figure 17]. Thus, the formulation can be said to be stable. The stability may be attributed to the polymers.
Figure 17: Stability study of optimized formulation

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  Conclusion Top

Carbopol and HPMC K100M can be promising polymers for gastroretentive bioadhesive drug delivery system. High bioadhesive strength of the formulation is likely to increase its GI residence time, and eventually improve the extent of bioavailability. Swelling studies indicated significant water uptake and contributed in drug release; swelling could also help in gastroretention. The optimized formulation sustained the release up to 24 h, followed Higuchi kinetics, while the drug release mechanism was found to be of anomalous type, and controlled by diffusion through the swollen matrix. Sustained drug release with high bioadhesive strength was observed in case of optimized formulation. The swollen tablet also maintained its physical integrity during the drug release test. The optimized formulation was found to stable in short-term accelerated stability testing at 40°C at 75% RH for a period of 1 month.

  References Top

1.Chavanpatil MD, Jain P, Chaudhari S, Shear R, Vavia P. Development of sustained release gastroretentive drug delivery system for ofloxacin: In vitro and In vivo evaluation. Int J Pharm 2005;304:178-184.  Back to cited text no. 1
2.Ponchel G, Irache J. Specific and non-specific bioadhesive particulate systems for oral delivery to the gastrointestinal tract. Adv Drug Deliv Rev 1998;34:191-219.  Back to cited text no. 2
3.Peppas NA, Sahlin JJ. Hydrogels as mucoadhesive and bioadhesive materials a review. Biomaterials 1996;17:1553-61.  Back to cited text no. 3
4.Hou SY, Cowles VE, Berner B. Gastric retentive dosage forms: A review. Crit Rev Ther Drug Carrier Syst 2003;20:459-97.  Back to cited text no. 4
5.Lavelle EC. Targeted delivery of drugs to the gastrointestinal tract. Crit Rev Ther Drug Carrier Syst 2001;18:341-86.  Back to cited text no. 5
6.Woodley J. Bioadhesion: New possibilities for drug administration? Clin Pharmacokinet 2001;40:77-84.  Back to cited text no. 6
7.Chavanpatil MD, Jain P, Chaudhari S, Shear, Vavia PR. Novel sustained release, swellable and bioadhesive gastroretentive drug delivery system for ofloxacin. Int J Pharm 2006;316:86-92.  Back to cited text no. 7
8.British Pharmacopoeia. Cambridge: University Printing House; 2002.  Back to cited text no. 8
9.Block JH, Beale JM. Wilson and Gisvold's Textbook of Organic Medicinal and Pharmaceutical Chemistry. 11 th ed. Philadelphia: Lippincott Williams and Wilkins;2004.  Back to cited text no. 9
10.Dyer JR. Applications of Absorption Spectroscopy of Organic Compounds. 1 st ed.New Delhi: Prentice-Hall of India Pvt. Ltd.1997.  Back to cited text no. 10
11.Indian Pharmacopoeia, Government of India Ministry of Health and Family Welfare. Vol. 1 and 3. 5th ed. The Indian Pharmacopoeia commission:Ghaziabad;2007.   Back to cited text no. 11
12.Suryanarayanan R. X-ray powder diffractometry. In Brittain HG, editor. Physical characterization of pharmaceutical solids. New York: Marcel Dekker Inc; 1993.p. 188-217.  Back to cited text no. 12
13.Singh B, Chakkal SK, Ahuja N. Formulation and optimization of controlled release mucoadhesive tablets of atenolol using response surface methodology. AAPS Pharm Sci Tech 2006;7:E3.  Back to cited text no. 13
14.Patel VF, Patel NM. Intragastric floating drug delivery system of cefuroxime acetyl: In Vitro evaluation. AAPS Pharm Sci Tech 2006;7:E17.  Back to cited text no. 14
15.Bhalekar MR, Madgulkar AR, Sheladiya DD, Kshirsagar SJ, Wable ND, Desale SS. Statistical Optimization of sustained release venlafaxine HCl wax matrix tablet. Indian J Pharm Sci 2008;70:472-6.  Back to cited text no. 15
[PUBMED]  Medknow Journal  
16.Jaimini M, Rana AC, Tanwar YS. Formulation and evaluation of famotidine floating tablets. Curr Drug Deliv 2007;4:51-5.   Back to cited text no. 16
17.Lieberman HA, Lachman L. Pharmaceutical dosage forms tablets. New York: Marcel Dekker, Inc;1981.  Back to cited text no. 17
18.Lalla JK, Gurnancy RA. Polymers for mucosal delivery-swelling and mucoadhesive delivery. Indian Drugs 2002;39:270-6.  Back to cited text no. 18
19.Ahuja S, Ahuja A, Baboota S, Ali J. Dissolution a promising tool in drug delivery. Indian J Pharm Sci 2005;67:650-60.  Back to cited text no. 19
  Medknow Journal  
20.Costa P, Sousa Lobo JM. Modeling and comparison of dissolution profiles. Eur J Pharm Sci 2001;13:123-33.  Back to cited text no. 20
21.Colombo P, Santi P, Bettini R, Brazel CS, Peppas NA. Drug release from swelling-controlled system. In. Wise DL, ed. Handbook of pharmaceutical controlled release technology. 1st Indian reprint; New York: Marcel Dekker, Inc; 2005. p. 183-209.  Back to cited text no. 21
22.Costa P. An alternative method to the evaluation of similarity factor in dissolution testing. Int J Pharm 2001;220:77-83.  Back to cited text no. 22
23.Jain KS, Agarwal GP, Jain NK. A novel calcium silicate microsphere of repaglinide in vivo investigation. J Control Release 2006;113:111-6.  Back to cited text no. 23
24.Mazzo DJ. International stability testing. USA:Interpharm press, Inc; 2005.  Back to cited text no. 24
25.Jamzad S, Tutunji L, Fassihi R. Analysis of macromolecular changes and drug release from hydrophilic matrix systems. Int J Pharm 2005;292:75-85.  Back to cited text no. 25
26.Hong SI, Oh SY. Dissolution kinetics and physical characterization of three-layered tablet with poly (ethylene oxide) core matrix capped by Carbopol. Int J Pharm 2008;356:121-9.  Back to cited text no. 26


  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8], [Figure 9], [Figure 10], [Figure 11], [Figure 12], [Figure 13], [Figure 14], [Figure 15], [Figure 16], [Figure 17]

  [Table 1], [Table 2], [Table 3], [Table 4], [Table 5], [Table 6], [Table 7], [Table 8], [Table 9], [Table 10], [Table 11], [Table 12], [Table 13]

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