Users Online: 374 | Home Print this page Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL RESEARCH ARTICLE
Year : 2011  |  Volume : 1  |  Issue : 2  |  Page : 119-125

Preparation and characterization of albumin nanoparticles encapsulating curcumin intended for the treatment of breast cancer


1 Department of Pharmaceutics, Mother Teresa College of Pharmacy, Ghatkesar, Hyderabad, India
2 Care College of Pharmacy, Kakatiya University, Warangal - 506 009, India
3 Vagdevi College of Pharmacy, Kakatiya University, Warangal - 506 009, India
4 University College of Pharmaceutical Sciences, Kakatiya University, Warangal - 506 009, India

Correspondence Address:
A V Jithan
Mother Teresa College of Pharmacy, NFC Nagar, Ghatkesar, Hyderabad
India
Login to access the Email id

Source of Support: SERC-DST, Conflict of Interest: None


DOI: 10.4103/2230-973X.82432

Rights and Permissions

Introduction: For the real-time clinical utilization of curcumin (an ayurvedic natural product) to treat breast cancer, its dissolution, rate limited solubility, poor tissue absorption, and extensive in vivo metabolism that leads to its poor systemic bioavailability should be overcome. A polymer-based nanoparticle formulation using bovine serum albumin can increase its aqueous solubility and can achieve protected, sustained, and targeted therapy in breast cancer. Materials and Methods: Desolvation technique was optimized for the preparation of albumin nanoparticles. Particle size, drug release, encapsulation efficiency, drug polymer interaction were the in vitro properties that were determined. Cell culture studies, in vivo pharmacokinetics in rats were used for biological characterization of the formulation. Results: The formulations were successfully prepared using 1:1, 1:2, 1:3, 1:4 drug: polymer ratios and the percent entrapment was found to be 74.76%, 91.01%, 85.36%, 86.42%, respectively, and particle size determined by zetasizer was found to be 225.1, 223.5, 226.3, 228.7 nm, respectively, and in vitro release was sustained for at least one month with drug release of 75.74%, 65.97%, 64.42%, 54%, respectively. The dissolution rate and aqueous solubility of curcumin was enhanced with this formulation. Fourier transform infrared spectroscopy (FTIR) studies demonstrated that the drug was not changed in the formulation during the fabrication process. The proliferation assays in MDA-MB-231 tumor cell lines indicated more effectiveness of the formulation compared to its solution form. In rats, albumin nanoparticles sustained drug release, demonstrated more bioavailability, improved pharmacokinetic properties, and enhanced tissue targetability of the drug. Conclusions: An effective curcumin-albumin nanoparticle formulation was successfully developed using a desolvation technique.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed7522    
    Printed342    
    Emailed4    
    PDF Downloaded1708    
    Comments [Add]    
    Cited by others 33    

Recommend this journal